u2932 cell line  (DSMZ)

Journal: Cell Communication and Signaling : CCS

Article Title: ORY1001 enhances anti-tumor immunity via CXCL10-mediated macrophage modulation in EBV-positive DLBCL

doi: 10.1186/s12964-025-02614-0

Figure Lengend Snippet: LSD1 inhibition promotes M1-like polarization of monocyte-derived macrophages. A Riva cells expressing either control vector or LMP1 were treated with ORY1001 (10 µM) for 24 or 48 h, and the conditioned media were collected. The media were added to THP-1-derived monocytes and cultured for 72 h. The expression of M1 and M2 macrophage markers was assessed by qPCR. B Under the same conditions, the expression of M1 and M2 markers was validated by flow cytometry. C-D CD14+ monocytes were isolated from PBMCs and differentiated into M0 macrophages. These cells were then cultured with conditioned media from ORY1001-treated Riva cells, followed by analysis of M1/M2 marker expression using qPCR ( C ) and flow cytometry D . E–F To assess whether these effects were conserved in additional EBV-related models, LMP1-expressing U2932 cells and primary EBV+ DLBCL cells were treated with ORY1001 (10 µM), and conditioned media were collected and applied to THP-1-derived monocytes or primary CD14+ monocytes. After 72 h of culture, M1- and M2-associated markers were quantified by qPCR ( E ), and surface expression of CD80 and CD163 was analyzed by flow cytometry F . Significant differences between control and treated groups are indicated as * ( p < 0.05), ** ( p < 0.01) and *** ( p < 0.001). Statistical significance for 24-hour treatments was evaluated using unpaired two-tailed t-test

Article Snippet: The Riva and U2932 cell lines were obtained from the Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (Braunschweig, Germany).

Techniques: Inhibition, Derivative Assay, Expressing, Control, Plasmid Preparation, Cell Culture, Flow Cytometry, Isolation, Marker, Two Tailed Test

Journal: Scientific Reports

Article Title: Anti-cancer activity of propolis extracts from stingless bees of Brunei Darussalam

doi: 10.1038/s41598-025-32628-0

Figure Lengend Snippet: Preliminary single dose treatment (50 µg/mL) with stingless bee propolis extracts and its effect on cancer cell viability. ( A ) Comparison of the effects of ethanol and ethyl acetate extracts from five stingless bee species on the viability of three cancer cell lines (U2932, H1975, and EJ). Statistical significance is indicated. ***, p < 0.001; **, p < 0.01; *, p < 0.05; n.s., non-significant. ( B ) Comparison of the effects of ethanol propolis extracts (50 µg/mL) from five stingless bee species ( H. itama , T. melanoleuca , T. binghami , G. thoracica , and L. canifrons ) on the viability of five cancer cell lines (U2932, H1975, OCI-LY3, HT, and EJ). The heatmap represents post-treatment cell viability (%), where lower values (red) indicate stronger cytotoxic effects.

Article Snippet: DLBCL U2932 cell line (Catalog no. ACC 633, DSMZ, Germany) were maintained in modified RPMI supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin-streptomycin.

Techniques: Comparison

Journal: Scientific Reports

Article Title: Anti-cancer activity of propolis extracts from stingless bees of Brunei Darussalam

doi: 10.1038/s41598-025-32628-0

Figure Lengend Snippet: Dose-dependent and time-dependent response of stingless bee ethanol extracts on cancer cell lines. ( A ) Heatmap of IC50 values across cell lines (EJ, H1975, U2932, HT, and OCI-LY3) and bee species ethanol extracts ( T. melanocephala , H. itama , L. canifrons , G. thoracica , and T. binghami ). Lower IC50 values (represented by darker blue) indicate higher potency. ( B ) Time-dependent response to extracts from T. melanoleuca and H. itama in U2932 and H1975 cell lines, as well as healthy PBMC. ( C ) H1975 and U2932 cell lines treated with osimertinib and doxorubicin.

Article Snippet: DLBCL U2932 cell line (Catalog no. ACC 633, DSMZ, Germany) were maintained in modified RPMI supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin-streptomycin.

Techniques:

Journal: Scientific Reports

Article Title: Anti-cancer activity of propolis extracts from stingless bees of Brunei Darussalam

doi: 10.1038/s41598-025-32628-0

Figure Lengend Snippet: Pathway enrichment following treatment of propolis ethanol extracts. Dot plots showing enriched cancer hallmark pathways based on differentially expressed genes following treatment with propolis ethanol extracts from three stingless bee species— H. itama , T. binghami , and T. melanoleuca . Dot size corresponds to the gene ratio, while color intensity represents the significance of enrichment (adjusted p-value). Pathways are grouped by cell line, illustrating upregulated (up) and downregulated (down) expression patterns across treatments. ( A ) Significant results shown for EJ, H1975, and HT cell lines. ( B ) Significant results shown for U2932 cell line. ( C ) Western blot showing corroborative evidence for p53 pathway activation in H1975 cell line, and apoptosis induction in U2932 cell line.

Article Snippet: DLBCL U2932 cell line (Catalog no. ACC 633, DSMZ, Germany) were maintained in modified RPMI supplemented with 10% fetal bovine serum, 1% L-glutamine, and 1% penicillin-streptomycin.

Techniques: Expressing, Western Blot, Activation Assay

Journal: Cancer Biology & Therapy

Article Title: FASN contributes to ADM resistance of diffuse large B-cell lymphoma by inhibiting ferroptosis via nf-κB/STAT3/GPX4 axis

doi: 10.1080/15384047.2024.2403197

Figure Lengend Snippet: FASN promoted tumor growth and resistance of DLBCL to ADM by inhibiting ferroptosis in vivo . The mice ( n = 6) were injected with FASN overexpressed or the control SU-DHL-2 cells subcutaneously to establish xenograft mice models. The mice were randomly divided into 4 groups, and were injected intraperitoneally with 2 mg/kg ADM twice a week or 20 mg/kg erastin every two days, as indicated by grouping. (a) The xenograft tumor tissues were isolated from the mice. (b) The weight and volume of the tumors were recorded and quantified. (c) The expression of GPX4, FASN, and 4-HNE in tumor tissues was validated by the IHC experiment. (d) The cell apoptosis of tumor tissues was detected by the TUNEL assay. Data from at least three repeated experiments. * p <.05, ** p <.01.

Article Snippet: The two DLBCL cell lines (SU-DHL-2 and U2932 cells) obtained from DSMZ (German Collection of Microorganisms and Cell Cultures) were cultured in RPMI-1640 medium (Tecno, Hangzhou, China, L121–500) with 10% FBS (SERANA, Brandenburg, Germany, S-FBS-SA-015) and 1% penicillin–streptomycin (Pen-Strep, Gibco, Semitic, NY, USA 15,140,122) added in a 5% CO 2 incubator at 37°C.

Techniques: In Vivo, Injection, Control, Isolation, Expressing, TUNEL Assay